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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. The two main areas of the clinical laboratory are:

A) cytology and histology.
B) urinalysis and microbiology.
C) chemistry and hematology.
D) anatomical and clinical.

2. Thin-layer chromatography has the advantage of being able to test for a large number of drugs at the same time.
Thin-layer chromatography is particularly useful as a tool in the identification of:

A) Enzymes
B) Hormones
C) Drugs
D) Steroids

3. Match each of the descriptions with the appropriate magnification:
1. Color, Rouleau, Overall Slide Quality, Cell Distribution
2. Platelet estimates RBC-platelet-WBC morphology WBC differential RBC inclusions
3. Select area to examine, WBC estimate

A) 10X
B) 100X (Oil)
C) 40X (Dry)

4. The laboratory is under the direction of a:

A) phlebotomist
B) pharmacist
C) medical assistant
D) pathologist

5. The clue to the identification of the colony seen in the upper frame is the gram stain in the lower frame, in which are seen short, rounded, gram positive bacilli, many of which possess distinct spores. As the colonies grew aerobically, the presence of spores indicates Bacillus species. The colonies are spreading, smooth, yellow-white and non-hemolytic. The lack of hemolysis and the small size of the bacterial cells suggests a species other than Bacillus cereus, the species causing most human infections. The isolate was identified as Bacillus circulans, which is consistent with the gram stain morphology. Clostridium septicum also produces spores; however, this species is an anaerobe and would grow poorly if at all and not produce spores aerobically. Listeria monocytogenes and Lactobacillus species are gram positive bacilli; however, neither of these produce spores.
The colonies seen in the upper frame, grown after 48 hours incubation aerobically at 35°C, and the accompanying gram stain in the lower frame are uncommonly associated with human disease, but have been associated with septicemia, bronchopneumonia, osteomyelitis and other infections, particularly in intravenous drug users. The most likely identification is:

A) Bacillus circulans
B) Clostridium septicum
C) Listeria monocytogenes
D) Lactobacillus species

Solutions: